By C. L. Ghai
With the writing of the eighth version of this famous and renowned publication on functional body structure, it has accomplished over 25 years of improvement. the scholars have constantly demanded simplicity, directness, and readability of expression that's inside of their effortless clutch. it truly is was hoping that the e-book meets with their necessities. The booklet conforms to the syllabi and classes mandated via the clinical and Dental Councils of India. As consistent with their new curriculum coverage, the sections on Hematology, Human Experiments, and medical exam, were vastly elevated and up-to-date and lots of new figures and diagrams additional. The part on Animal Experiments has been curtailed. The Appendix provides an enormous volume of Physiological info. a brand new characteristic has been the inclusion of OSPEs (Objective established functional Examinations) for assessing scholars’ ability in useful body structure examinations. intended basically for MBBS, BDS, B. Ph-Th scholars, the ebook will end up worthwhile to scholars of BSc and MSc (Physiology), BSc and MSc (Pharmacy), MD (Physiology) and people learning Human body structure in lifestyles Sciences schools and Universities.
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Additional info for A Textbook of Practical Physiology
Describe the possible sources of error during dilution of blood. Hemocytometry Diluting pipettes Principle. Parts of a diluting pipette. Units for reporting Principle underlying their use. Sources of error. Differences between RBC and WBC pipettes. Steps in hemocytometry Filling the pipette with blood and diluting it. Hemocytometer Calculation of dilution obtained. Diluting pipettes Precautions. Counting chamber Questions HEMOCYTOMETRY Hemocytometry is the procedure of counting the number of cells in a sample of blood; the red cells, the white cells, and the platelets being counted separately.
Alternately, a nick may be given with the corner edge of a sterile blade to obtain blood. (The BT and CT tests give better results here than at the finger-prick). Pricking the Heel In infants and young children, blood can be collected from the cleaned and warmed medial or lateral areas of the heel. The central plantar and the posterior curvature areas of the heel should be avoided as the prick may cause injury to the underlying tarsal bones which lie near the surface. PRECAUTIONS 1. Keep the equipment for the test ready before getting/giving a finger prick 2.
Counting the cells and reporting the results. Figure 1-6 shows the two glass capillary pipettes used for diluting the blood. Each pipette has a long narrow stem (for measuring the blood), which widens into HEMOCYTOMETER The hemocytometer set consists of the following: 1. 0, and 11 (see text for dilutions obtained) 25 Hematology a bulb (for diluting the blood/which in turn, leads to a short stem. Parts of a Diluting Pipette The stem. The long narrow stem has a capillary bore and a well-grounded conical tip.
A Textbook of Practical Physiology by C. L. Ghai